Microtubule

Microtubule - Super resolution NBI

Two-dimensional super resolution imaging of microtubules in COS-7 cell. Beta-tubulin was detected using indirect immunostaining. Microtubule filaments were clearly separated even in the dense area in super resolution image.

Mitochondria

mitochondria - Super resolution NBI

Three-dimensional super resolution image of mitochondria in COS-7 cell. The mitochondria outer membrane protein Tom20 was detected using indirect immunostaining. The mitochondrial outer membrane appeared as a thin envelope that enclosed a hollow space, which is typically difficult to resolve by conventional wide-field or confocal fluorescence microscopy. The 3d depth information is color-coded.

Golgi Apparatus

Golgi Apparatus NBI Nanobioimaging super rsolution Two-color super resolution imaging resolves GM130 (cis-Golgi matrix protein) and Golgin97 (trans-Golgi network membrane protein) in HeLa cell line. (a) Epi fluorescent image with totally blurred strucutre and more “overlapping” regions of the two proteins. (b) The super resolution images at the same location. The two layers (cis vs. trans) in golgi apparatus were better distinguished.

(Collaboration with biochemistry group from HKUST)

Mitochondrial Membrane Structure

Mitochondrial Membrane Structure NBI Nanobioimaging super rsolution Two-color super resolution imaging resolves p32 (mitochondrial matrix) packed by Tom20 (mitochondrial outer membrane) in HEK293 cell line. (a) Epi fluorescent image with totally blurred structure. (b) The super resolution images at the same location. (c) The transvers profile correspondence to boxed region in (b), showing clear the p32 is packed inside mitochondrial outer membrane. Scale bars: 1μm.

(Collaboration with biochemistry group from HKUST)

Neuron Synapse

Neuron Synapse NBI Nanobioimaging super rsolution TSynaptic structure in tissue slice resolved by localization microscope. (a)(c) and (e) show the EPI-fluorescent image which fails to tell the details of structure. (b)(d) and (f) are superresolution images acquired by NBI two-color localization microscope. The pre- and post-synaptic structures are clearly resolved.

(Collaboration with biochemistry group from HKUST)

Yeast Organelle

Yeast Organelle NBI Nanobioimaging super rsolution Two-color super resolution imaging of Alexa750-anti-GFP nanobody labeled Tub1 of spindle microtubule and Alexa647-anti-RFP nanobody labeled Tub4 of spindle pole body in a yeast cell. (a) and (b) are Epi fluorescent images where (b) is the zoom in of the marked area in (a). (c) and (d) are the two-color super resolution images at the same position where the anchoring of spindle microtubules to spindle pole bodies can be clearly resolved. Scale bar: 1μm in (a) and (c); 200nm in (b) and (d).

(Collaboration with biochemistry group from HKUST)

3d clathrin-coated-pits

3d clathrin NBI Nanobioimaging super rsolution

Clathrin-coated-pits (one type of vesicle) is visualized in COS-7 cells. Multiple vesicles were zoomed in and the donut structure with diameter of around 200 nm were shown. The 3d depth information is color-coded.

3d Actin

3d Actin NBI Nanobioimaging super rsolution

Actin fibers in COS-7 cell is super-resolved in three dimensions. Actin is stained with phalloidin and the fine actin fiber (7nm-wide) can be visualized. It allows the detection of actin network modification. The 3d depth information is color-coded.